PAK-1,2,3 (Thr402) Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-70213r |
| Product Name | PAK-1,2,3 (Thr402) Antibody |
| Applications |
WB
|
| Specificity | Specific for endogenous levels of the ~68 kDa PAK protein phosphorylated at Thr402. The immunolabeling is completely eliminated by treatment with λ-phosphatase. The peptide sequence used is identical in PAK-1, 2 and 3. Note: Thr402 in PAK-2 corresponds to Thr423 in human PAK-1. |
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Dog, Bovine |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr402 of rat p21 activated kinase 2 (PAK-2), conjugated to keyhole limpet hemocyanin (KLH). |
| Modification Site | Thr402 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Antigen Affinity purification from Pooled whole antiserum |
| Storage Buffer | 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. |
| Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target | |
| Gene ID | 29431 |
| Swiss Prot | P35465 |
| Synonyms | Alpha PAK antibody, Beta PAK antibody, bPAK antibody, CDKN1A antibody, Gamma PAK antibody, hPAK3 antibody, MRX30 antibody, MRX47 antibody, Oligophrenin 3 antibody, OPHN3 antibody, P21 (CDKN1A) activated kinase 2 antibody, P21 (CDKN1A) activated kinase 3 antibody, p21 activated kinase 1 antibody, p21 activated kinase 2 antibody, p21 activated kinase 3 antibody, P21 protein (Cdc42/Rac) activated kinase 1 antibody, P21 protein (Cdc42/Rac) activated kinase 2 antibody, P21 protein (Cdc42/Rac) activated kinase 3 antibody, P21/Cdc42/Rac1 activated kinase 1 (STE20 homolog yeast) antibody, P21/Cdc42/Rac1 activated kinase 1 (yeast Ste20 related) antibody, P58 antibody, P65 PAK antibody, PAK 2 antibody, PAK 3 antibody, PAK1 antibody, PAK2 antibody, PAK3 antibody, PAK3beta antibody, PAK65 antibody, PAKalpha antibody, PAKgamma antibody, S6/H4 kinase antibody, Serine/threonine protein kinase PAK 1 antibody, Serine/threonine protein kinase PAK 2 antibody, Serine/threonine protein kinase PAK 3 antibody, Serine/threonine protein kinase PAK1 antibody, Serine/threonine protein kinase PAK2 antibody, Serine/threonine protein kinase PAK3 antibody, STE20 homolog yeast antibody |
| Background | In mammals, there are several identified isoforms of p21-activated protein kinases or PAKs: α-PAK (also known as PAK-1) and β-PAK (also known as PAK-3) are mostly brain-specific, while γ-PAK (also known as PAK-2) is expressed ubiquitously (Jakobi et al., 2003). Mutations of the gene coding for PAK-3 are associated with X-linked mental retardation and recent work indicates that PAK-3 is a key regulator of synapse formation and plasticity in the hippocampus (Boda et al., 2004). PAK-3 is thought to play a key role in regulation of cell shape and motility as well as cell death (Jakobi et al., 2003; Walter et al., 1998). Autophosphorylation of Thr-402 in the protein has been found to be essential for activation of PAK (Jakobi et al., 2000). |
| Application Dilution | |
| WB | 1:300-5000 |