Potassium Chloride Cotransporter (KCC2) (Ser940) Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-70228r-100uL |
| Product Name | Potassium Chloride Cotransporter (KCC2) (Ser940) Antibody |
| Applications |
WB
IP
|
| Specificity | Specific for endogenous levels of the ~135 kDa KCC2 protein phosphorylated at Ser940. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide. |
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Dog, Bovine, Zebrafish, Non-Human Primate |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser940 of rat KCC2, conjugated to keyhole limpet hemocyanin (KLH). |
| Modification Site | Ser940 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Antigen Affinity purification from Pooled whole antiserum |
| Storage Buffer | 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. |
| Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target | |
| Gene ID | 171373 |
| Swiss Prot | Q63633 |
| Synonyms | Electroneutral potassium chloride cotransporter 2 antibody, Electroneutral potassium-chloride cotransporter 2 antibody, Erythroid K Cl cotransporter 2 antibody, Furosemide sensitive K Cl cotransporter antibody, hKCC2 antibody, K-Cl cotransporter 2 antibody, KCC 2 antibody, KCC2 antibody, KIAA1176 antibody, Neuronal K Cl cotransporter antibody, Neuronal K-Cl cotransporter antibody, Potassium Chloride Cotransporter antibody, Potassium chloride transporter 5 antibody, rKCC2 antibody, S12A5 antibody, S12A5_HUMAN antibody, SLC12A5 antibody, Solute carrier family 12 (potassium chloride transporter) member 5 antibody, Solute carrier family 12 member 5 antibody |
| Background | KCC2 is widely thought to be expressed exclusively in neurons where it is responsible for maintaining low intracellular chloride concentration to drive hyperpolarizing post-synaptic responses to the inhibitory neurotransmitters GABA and glycine. Serine 940 on KCC2 has been shown to be phosphorylated by PKC and has further been demonstrated to be the major site for PKC-dependent phosphorylation for full length KCC2 molecules when expressed in HEK-293 cells as phosphorylation of Ser-940 increased the cell surface stability of KCC2 in this system by decreasing it’s rate of internalization from the plasma membrane (Lee et al., 2007). |
| Application Dilution | |
| WB | 1:300-5000 |
| IP | 1-2ug |
Frequently asked questions
Which applications is Potassium Chloride Cotransporter (KCC2) (Ser940) Antibody (bs-70228R) validated for, and at what dilution?
bs-70228R is validated for WB (1:300-5000); IP (1-2ug). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Potassium Chloride Cotransporter (KCC2) (Ser940) Antibody (bs-70228R) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Bovine, Zebrafish, Non-Human Primate.
How should bs-70228R be stored?
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. Storage buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
What immunogen was used to raise Potassium Chloride Cotransporter (KCC2) (Ser940) Antibody (bs-70228R)?
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser940 of rat KCC2, conjugated to keyhole limpet hemocyanin (KLH).