PTPH1 (Ser459) Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-70236r |
| Product Name | PTPH1 (Ser459) Antibody |
| Applications |
WB
|
| Specificity | Specific for endogenous levels of the ~104 kDa PTPH1 protein phosphorylated at Ser459. The immunolabeling is completely eliminated by treatment with λ-phosphatase. |
| Reactivity | Human, Mouse |
| Predicted Reactivity | Dog, Bovine, Sheep, Non-Human Primate |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser459 of human PTPH1, conjugated to keyhole limpet hemocyanin (KLH). |
| Modification Site | Ser459 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Antigen Affinity purification from Pooled whole antiserum |
| Storage Buffer | 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. |
| Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target | |
| Gene ID | 5774 |
| Swiss Prot | P26045 |
| Synonyms | cytoskeletal-associated protein tyrosine phosphatase antibody, protein tyrosine phosphatase H1 antibody, protein tyrosine phosphatase non-receptor type 3 antibody, Protein-tyrosine phosphatase H1 antibody, PTN3_HUMAN antibody, PTP-H1 antibody, PTPH1 antibody, PTPN3 antibody, Tyrosine-protein phosphatase non-receptor type 3 antibody |
| Background | Protein-tyrosine Phosphatase H1 (PTPH1) has recently been identified as a specific p38γ MAPK phosphatase which is mediated through PDZ interaction (Hou et al., 2010). Ras has been demonstrated to increase both p38γ and PTPH1 protein expression, and there is a coupling of increased p38γ and PTPH1 protein expression in primary colon cancer tissues (Hou et al., 2010). Phosphorylation of PTPH1 at Ser-459 leads to PTPH1 stabilization, which plays an important role in Ras oncogenesis and stress response (Hou et al., 2012). Additionally, phosphorylation of PTPH1 Ser-459 reveals a novel mechanism by which MAPK signals through PTPH1 to regulate cellular response (Hou et al., 2012). |
| Application Dilution | |
| WB | 1:300-5000 |