Interferon alpha 11 Polyclonal Antibody, Cy5 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-7023R-Cy5 |
| Product Name | Interferon alpha 11 Polyclonal Antibody, Cy5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Specificity | This antibody will detect all Human and Mouse Interferon alpha subtypes |
| Predicted Reactivity | Mouse, Rat |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from Mouse IFNA1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 15962 |
| Swiss Prot | P01572 |
| Subcellular location | Secreted |
| Synonyms | IFNA; IFN-a; IFN-_; Interferon alpha; Interferon-alpha; IFNA1; IFNA2; IFNA5; IFNA6; IFNA8/6; IFNA7; IFNA9; IFNA11; IFNA12; IFNA13; IFNA15; IFNA16; IFNA6T; IFNAB; IFNA4; Pan Interferon alpha; IFNA13; IFNA1/13; IFNA7; IFNA10; IFNA14; IFNA17; IFNA21; IFNA8. |
| Background | Interferons(IFNs) are proteins made and released by host cells in response to the presence of pathogens such as viruses, bacteria, parasites or tumor cells. They belong to the large class of glycoproteins known as cytokines. IFNs stimulate the production of two enzymes: a protein kinase and an oligoadenylate synthetase. They allow for communication between cells to trigger the protective defenses of the immune system that eradicate pathogens or tumors. IFNs can activate immune cells, such as natural killer cells and macrophages; they increase recognition of infection or tumor cells by up-regulating antigen presentation to T lymphocytes; and they also increase the ability of uninfected host cells to resist new infection by virus. Leukocyte interferon is produced predominantly by B lymphocytes. Immune interferon is produced by mitogen- or antigen-stimulated T lymphocytes. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Interferon alpha 11 Polyclonal Antibody, Cy5 Conjugated (bs-7023R-Cy5) validated for, and at what dilution?
bs-7023R-Cy5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-7023R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Interferon alpha 11 Polyclonal Antibody, Cy5 Conjugated (bs-7023R-Cy5)?
KLH conjugated synthetic peptide derived from Mouse IFNA1
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-7023R-Cy5)?
Cy5 on bs-7023R-Cy5: excitation / emission 625,650nm/670nm.