Synapsin (Ser549) Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-70269r |
| Product Name | Synapsin (Ser549) Antibody |
| Applications |
WB
|
| Specificity | Specific for endogenous levels of the ~78 kDa synapsin I doublet phosphorylated at Ser549. Immunolabeling is completely eliminated by treatment with λ-phosphatase. |
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Dog, Bovine, Non-Human Primate |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser549 of rat synapsin, conjugated to keyhole limpet hemocyanin (KLH). |
| Modification Site | Ser549 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Antigen Affinity purification from Pooled whole antiserum |
| Storage Buffer | 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. |
| Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target | |
| Gene ID | 281510 |
| Swiss Prot | P17599 |
| Synonyms | Brain protein 4.1 antibody, SYN 1 antibody, SYN 1a antibody, SYN 1b antibody, SYN I antibody, SYN1 antibody, SYN1_HUMAN antibody, SYN1a antibody, SYN1b antibody, Synapsin 1 antibody, Synapsin I antibody, Synapsin-1 antibody, Synapsin1 antibody, SynapsinI antibody, SYNI antibody |
| Background | Synapsin I plays a key role in synaptic plasticity in brain (Feng et al., 2002; Nayak et al., 1996). This effect is due in large part to the ability of the synapsins to regulate the availability of synaptic vesicles for release. The role of synapsin in synaptic plasticity and in synaptogenesis is regulated by phosphorylation (Jovanovic et al., 2001; Kao et al., 2002). Ser-549 along with Ser-62 and Ser-67 are the sites of synapsin I that are phosphorylated by MAP kinase (Jovanovic et al., 1996). Phosphorylation and subsequent dephosphorylation of this site is thought to play a key role in synaptic vesicle trafficking. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is Synapsin (Ser549) Antibody (bs-70269R) validated for, and at what dilution?
bs-70269R is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Synapsin (Ser549) Antibody (bs-70269R) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Bovine, Non-Human Primate.
How should bs-70269R be stored?
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. Storage buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
What immunogen was used to raise Synapsin (Ser549) Antibody (bs-70269R)?
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser549 of rat synapsin, conjugated to keyhole limpet hemocyanin (KLH).