Synaptotagmin 1 Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-70274r-100uL |
| Product Name | Synaptotagmin 1 Antibody |
| Applications |
WB
|
| Specificity | Specific for endogenous levels of the ~65 kDa synaptotagmin I protein. |
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Bovine, Chicken, Non-Human Primate |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Synthetic peptide corresponding to amino acid residues specific to rat synaptotagmin 1, conjugated to keyhole limpet hemocyanin (KLH). |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Antigen Affinity purification from Pooled whole antiserum |
| Storage Buffer | 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. |
| Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target | |
| Gene ID | 25716 |
| Swiss Prot | P21707 |
| Synonyms | DKFZp781D2042 antibody, FLJ42519 antibody, P65 antibody, SVP65 antibody, synaptotagmin 1 antibody, Synaptotagmin I antibody, SYT antibody, SYT1 antibody, SytI antibody |
| Background | Synaptotagmin 1 is a synaptic vesicle membrane glycoprotein that is widely expressed throughout the CNS and is generally thought to act as the Ca2+ sensor in the regulation of exocytosis and neurotransmitter release (Littleton and Bellen 1995). Recent studies indicate that synaptotagmin’s Ca2+ mediated binding of SNAP25 is essential for the Ca2+ dependent triggering of membrane fusion (Zhang et al., 2002). It has recently been demonstrated that discrete residues within the c(2)b binding domain of synaptotagmin 1 independently specify endocytic rate and synaptic vesicle size (Poskanzer et al., 2006). |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is Synaptotagmin 1 Antibody (bs-70274R) validated for, and at what dilution?
bs-70274R is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Synaptotagmin 1 Antibody (bs-70274R) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Bovine, Chicken, Non-Human Primate.
How should bs-70274R be stored?
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. Storage buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
What immunogen was used to raise Synaptotagmin 1 Antibody (bs-70274R)?
Synthetic peptide corresponding to amino acid residues specific to rat synaptotagmin 1, conjugated to keyhole limpet hemocyanin (KLH).