Synaptotagmin (Thr202) Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-70275r-100uL |
| Product Name | Synaptotagmin (Thr202) Antibody |
| Applications |
WB
|
| Specificity | Specific for endogenous levels of the ~62 kDa synaptotagmin protein phosphorylated at Thr 202. Immunolabeling is completely eliminated by treatment with λ-phosphatase. |
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse, Dog, Bovine, Chicken, Zebrafish, Non-Human Primate |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr202 of rat synaptotagmin, conjugated to keyhole limpet hemocyanin (KLH). |
| Modification Site | Thr202 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Antigen Affinity purification from Pooled whole antiserum |
| Storage Buffer | 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. |
| Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target | |
| Gene ID | 25716 |
| Swiss Prot | P21707 |
| Synonyms | DKFZp781D2042 antibody, FLJ42519 antibody, P65 antibody, SVP65 antibody, synaptotagmin 1 antibody, Synaptotagmin I antibody, SYT antibody, SYT1 antibody, SytI antibody |
| Background | Synaptotagmin is widely regarded as the primary calcium sensor for synaptic vesicle exocytosis (Fernandez-Chacon et al., 2001; Wang et al., 2003). Moreover, recent studies indicate that the protein also plays a key role in endocytosis (Poskanzer et al., 2003). Synaptotagmin can be phosphorylated by multiple protein kinases and this may play a key role in modulation of synaptotagmin’s ability to influence both the exocytotic and endocytotic components of synaptic transmission (Hilfiker et al., 1999; Lee et al., 2004). |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is Synaptotagmin (Thr202) Antibody (bs-70275R) validated for, and at what dilution?
bs-70275R is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Synaptotagmin (Thr202) Antibody (bs-70275R) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Dog, Bovine, Chicken, Zebrafish, Non-Human Primate.
How should bs-70275R be stored?
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. Storage buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
What immunogen was used to raise Synaptotagmin (Thr202) Antibody (bs-70275R)?
Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr202 of rat synaptotagmin, conjugated to keyhole limpet hemocyanin (KLH).