TAO2 (Ser181) Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-70278r-100uL |
| Product Name | TAO2 (Ser181) Antibody |
| Applications |
WB
|
| Specificity | Specific for endogenous levels of the ~120 kDa TAO2 phosphorylated at Ser181. Immunolabeling is completely eliminated by treatment with λ-phosphatase. |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Chicken, Zebrafish, Xenopus, Finch |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser181 of human TAO2, conjugated to keyhole limpet hemocyanin (KLH). |
| Modification Site | Ser181 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Antigen Affinity purification from Pooled whole antiserum |
| Storage Buffer | 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. |
| Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target | |
| Gene ID | 9344 |
| Swiss Prot | Q9UL54 |
| Synonyms | 1110033K02Rik antibody, B230344N16 antibody, hKFC C antibody, hKFC-C antibody, KIAA0881 antibody, Kinase from chicken homolog C antibody, MAP3K17 antibody, mKIAA0881 antibody, Prostate derived STE20 like kinase 1 antibody, Prostate derived STE20 like kinase PSK antibody, Prostate derived sterile 20 like kinase 1 antibody, Prostate-derived STE20-like kinase 1 antibody, PSK 1 antibody, PSK antibody, PSK-1 antibody, PSK1 antibody, PSK1 beta antibody, Serine/threonine protein kinase TAO2 antibody, Serine/threonine-protein kinase TAO2 antibody, TAO 1 antibody, TAO 2 antibody, TAO kinase 2 antibody, TAO1 antibody, TAO2 antibody, TAOK2 antibody, TAOK 2 antibody, Taok2 antibody, TAOK2_HUMAN antibody, Thousand and one amino acid protein 2 antibody, Thousand and one amino acid protein kinase antibody, UNQ2971/PRO7431 antibody |
| Background | In vitro, TAO (thousand and one amino acid) protein kinase 2 (TAO2) activates MAP/ERK kinases (MEKs) 3, 4, and 6 toward their substrates p38 MAP kinase JNK/SAPK (Chen et al., 1999; Chen and Cobb, 2001). This and more recent work has led to the proposal that the TAO protein kinases play an essential role in signaling from physiological agonists to the stress-responsive p38 MAPKs (Chen et al., 2003). Autophosphorylation of TAO may play a role in the mechanism of TAO activation. The MEK binding domain of TAO is autophosphorylated on both serine and threonine residues and Ser-181 is located within this domain. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is TAO2 (Ser181) Antibody (bs-70278R) validated for, and at what dilution?
bs-70278R is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does TAO2 (Ser181) Antibody (bs-70278R) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Chicken, Zebrafish, Xenopus, Finch.
How should bs-70278R be stored?
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. Storage buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
What immunogen was used to raise TAO2 (Ser181) Antibody (bs-70278R)?
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser181 of human TAO2, conjugated to keyhole limpet hemocyanin (KLH).