Tyrosine Hydroxylase Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-70303s-100uL |
| Product Name | Tyrosine Hydroxylase Antibody |
| Applications |
WB
IHC
|
| Specificity | Specific for endogenous levels of the ~60 kDa tyrosine hydroxylase protein. |
| Reactivity | Human, Mouse, Rat, All mammalian |
| Predicted Reactivity | Chicken |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Sheep |
| Source | SDS-denatured, native rat tyrosine hydroxylase purified from pheochromocytoma. |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Antigen Affinity purification from Pooled whole antiserum |
| Storage Buffer | 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. |
| Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target | |
| Gene ID | 25085 |
| Swiss Prot | P04177 |
| Synonyms | Dystonia 14 antibody, DYT14 antibody, DYT5b antibody, EC 1.14.16.2 antibody, OTTHUMP00000011225 antibody, OTTHUMP00000011226 antibody, ple antibody, Protein Pale antibody, TH antibody, The antibody, TY3H_HUMAN antibody, TYH antibody, Tyrosine 3 hydroxylase antibody, Tyrosine 3 monooxygenase antibody, Tyrosine 3-hydroxylase antibody, Tyrosine 3-monooxygenase antibody, Tyrosine hydroxylase antibody |
| Background | Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines dopamine and norepinephrine. TH antibodies can therefore be used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson’s disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999). TH antibodies can also be used to explore basic mechanisms of dopamine and norepinephrine signaling (Witkovsky et al., 2000; Salvatore et al., 2001). |
| Application Dilution | |
| WB | 1:300-5000 |
| IHC | |
Frequently asked questions
Which applications is Tyrosine Hydroxylase Antibody (bs-70303S) validated for, and at what dilution?
bs-70303S is validated for WB (1:300-5000); IHC. These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Tyrosine Hydroxylase Antibody (bs-70303S) react with?
Tested reactivity: Human, Mouse, Rat, All mammalian. Predicted reactivity (expected from sequence homology but not yet tested): Chicken.
How should bs-70303S be stored?
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. Storage buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
What immunogen was used to raise Tyrosine Hydroxylase Antibody (bs-70303S)?
SDS-denatured, native rat tyrosine hydroxylase purified from pheochromocytoma.