Vesicular GABA transporter (VGAT) Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-70309r |
| Product Name | Vesicular GABA transporter (VGAT) Antibody |
| Applications |
WB
IHC
|
| Specificity | Specific for endogenous levels of the ~53 kDa VGAT protein. |
| Reactivity | Mouse, Rat, Chicken, Xenopus |
| Predicted Reactivity | Dog, Bovine, Non-Human Primate |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Synthetic peptide corresponding to amino acid residues from the N-terminal region of rat VGAT, conjugated to keyhole limpet hemocyanin (KLH). |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Antigen Affinity purification from Pooled whole antiserum |
| Storage Buffer | 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. |
| Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target | |
| Gene ID | 83612 |
| Swiss Prot | O35458 |
| Synonyms | bA122O1.1 antibody, GABA and glycine transporter antibody, hVIAAT antibody, SLC32A1 antibody, SLC32A 1 antibody, Slc32a1 antibody, solute carrier family 32 (GABA vesicular transporter) member 1 antibody, Solute carrier family 32 member 1 antibody, Vesicular GABA Amino Acid Transporter antibody, Vesicular GABA transporter antibody, Vesicular inhibitory amino acid transporter antibody, VGAT antibody, VIAAT antibody, VIAAT_HUMAN antibody |
| Background | The Vesicular GABA Amino Acid Transporter (VGAT) is responsible for transport of the inhibitory neurotransmitter into synaptic vesicles(McIntire et al., 1997). The VGAT protein (also known as the Vesicular Inhibitory Amino Aid Transporter or VIAAT) is expressed in synaptic vesicles of both glycine and GABAergic synapses throughout the CNS (Chaudhry et al., 1998). Expression of the VGAT protein changes during development and also in response to patterns of neuronal activity (De et al., 2005). |
| Application Dilution | |
| WB | 1:300-5000 |
| IHC | |
Frequently asked questions
Which applications is Vesicular GABA transporter (VGAT) Antibody (bs-70309R) validated for, and at what dilution?
bs-70309R is validated for WB (1:300-5000); IHC. These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Vesicular GABA transporter (VGAT) Antibody (bs-70309R) react with?
Tested reactivity: Mouse, Rat, Chicken, Xenopus. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Bovine, Non-Human Primate.
How should bs-70309R be stored?
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. Storage buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
What immunogen was used to raise Vesicular GABA transporter (VGAT) Antibody (bs-70309R)?
Synthetic peptide corresponding to amino acid residues from the N-terminal region of rat VGAT, conjugated to keyhole limpet hemocyanin (KLH).