AIM2 (N-terminal region) Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bs-70328r |
| Product Name | AIM2 (N-terminal region) Antibody |
| Applications |
WB
|
| Specificity | The antibody detects a 40 kDa* doublet corresponding to AIM2 in immunoblots of Jurkat cells, as well as mouse macrophages treated with IFNγ and LPS. |
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | AIM2 synthetic peptide (coupled to KLH) corresponds to amino acid residues in the N-terminal region of human AIM2. This peptide sequence is highly conserved in rat and mouse AIM2. |
| Clonality | Monoclonal |
| Clone # | #REF! |
| Isotype | IgG |
| Purification | Antigen Affinity purification |
| Storage Buffer | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target | |
| Swiss Prot | O14862 |
| Synonyms | PYHIN4, AIM2 |
| Background | Host- and pathogen-associated cytoplasmic double-stranded DNA triggers the activation of a NALP3-independent inflammasome, which activates caspase-1, leading to maturation of pro-interleukin-1beta and inflammation. Several studies have isolated AIM2 (absent in melanoma 2) as a candidate cytoplasmic-DNA-sensing protein that contains an N-terminal pyrin domain and C-terminal oligonucleotide binding domain. A screen for transcripts induced by interferon-beta identified AIM2 gene expression. AIM2 protein bound double-stranded DNA, recruited the inflammasome adaptor ASC, and localized to ASC containing speckles. AIM2 and ASC form a pyroptosome, which induces pyroptotic cell death mediated by caspase-1. RNA-mediated suppression of AIM2 expression impairs DNA-induced maturation of interleukin-1beta in THP-1 human monocytic cells, as well as abrogates caspase-1 activation in response to cytoplasmic double-stranded DNA and the double-stranded DNA vaccinia virus. Thus, AIM2 is a DNA-sensing protein for the activation of the caspase-1 inflammasome. |
| Application Dilution | |
| WB | 1:300-5000 |