Arp2 (Thr-237/Thr-238), Phosphospecific Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bs-70345r |
| Product Name | Arp2 (Thr-237/Thr-238), Phosphospecific Antibody |
| Applications |
WB
|
| Specificity | The antibody detects 44 and 32 kDa* proteins corresponding to the molecular mass of Arp2 on SDS-PAGE immunoblots of human A431 and Jurkat cells treated with Calyculin A. This reactivity is not observed after lambda phosphatase treatment. This peptide sequence is highly conserved in rat, mouse, chicken, and fish Arp2 proteins. |
| Reactivity | Human, Mouse, Rat, Chicken |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Phospho-Arp2 (Thr-237/Thr-238) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding threonine 237 and threonine 238 in human Arp2. |
| Modification Site | T237/T238 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purification | Antigen Affinity purification |
| Storage Buffer | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target | |
| Swiss Prot | P61160 |
| Synonyms | ACTR2, Arp2 |
| Background | Cellular morphology, adhesion, and motility occur through dynamic reorganization of actin-based superstructures. Actin-binding proteins are critical for regulating actin polymerization and superstructure formation. The Arp2/3 complex is an actin polymerization-inducing complex that includes Arp2, Arp3, p41-Arc, p34-Arc, p21-Arc, p20-Arc, and p16-Arc. Several nucleation promoting factors, such as WASP and coronin, regulate the activity of the Arp2/3 complex. In addition, the Arp2/3 complex may be regulated by phosphorylation of specific subunits in the complex. Arp2 has two phosphosites, Thr-237 and Thr-238, that are evolutionarily conserved, and are phosphorylated along with Tyr-202 in response to growth factor stimulation. These phosphorylation events may regulate binding to the pointed end of actin filaments, and alanine substitutions of these Arp2 phosphosites inhibit membrane protrusions. Thus, phosphorylation may be another mode of Arp2/3 complex regulation in addition to the activity of nucleation-promoting factors. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is Arp2 (Thr-237/Thr-238), Phosphospecific Antibody (bs-70345R) validated for, and at what dilution?
bs-70345R is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Arp2 (Thr-237/Thr-238), Phosphospecific Antibody (bs-70345R) react with?
Tested reactivity: Human, Mouse, Rat, Chicken.
How should bs-70345R be stored?
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. Storage buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
What immunogen was used to raise Arp2 (Thr-237/Thr-238), Phosphospecific Antibody (bs-70345R)?
Phospho-Arp2 (Thr-237/Thr-238) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding threonine 237 and threonine 238 in human Arp2.