| Overview |
| bs-70378r |
| _1-Catenin (Tyr-148), Phosphospecific Antibody |
| WB |
| This antibody was cross-adsorbed to unrelated phospho-tyrosine peptide before affinity purification using phospho-_1-Catenin (Tyr-148) peptide (without carrier). The antibody detects a 102 kDa* protein corresponding to the molecular mass of _1-Catenin on SDS-PAGE immunoblots of rat PC12 cells treated with pervanadate. This peptide sequence is highly conserved in rat and mouse _1-Catenin, but is not conserved in _2-Catenin or _3-Catenin. |
| Human, Mouse, Rat, Chicken |
| Specifications |
| Unconjugated |
| Rabbit |
| Phospho-_1-Catenin (Tyr-148) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 148 in human _1-Catenin. |
| Tyr-148 |
| Polyclonal |
| #REF! |
| IgG |
| Antigen Affinity purification |
| PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage at -20C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20C. |
| Target |
| P35221 |
| alphaE-catenin, catenin alpha1, catenin |
| _-catenins are cadherin interacting proteins with homology to vinculin. Three _-catenin genes have been described including _1-catenin (_E-Catenin), _2-catenin (_N-catenin), and _3-catenin (_T-catenin). _1-catenin has 81% homology with _2-catenin and 60% homology with _3-catenin. These _-catenin isoforms may have similar roles since each binds cadherins. However, their expression patterns are both overlapping and distinct. _1-catenin was identified in epithelial cells, and is expressed in various cell types. _2-catenin is enriched in the nervous system, and _3-catenin is expressed highest in testis and heart. Phosphorylation may regulate the activity of _1-catenin, since tyrosine phosphorylation of Tyr-148 occurs during intercellular adhesion. This site is dephosphorylated by SHP2, which inhibits _1-catenin binding to _-catenin and translocation to the plasma membrane. Phosphorylation of _1-catenin at Tyr-148 may be important for inhibition of cell transformation, and dephosphorylation of this site may be important during SHP2-mediated cell transformation. |
| Application Dilution |
| WB |
1:300-5000 |
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