Caveolin-1 (N-terminal region) Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bs-70391r |
| Product Name | Caveolin-1 (N-terminal region) Antibody |
| Applications |
WB
|
| Specificity | This antibody detects a 21 kDa* protein corresponding to the apparent molecular mass of caveolin-1 on SDS-PAGE immunoblots of human A431 cells and rabbit spleen fibroblasts. |
| Reactivity | Human, Mouse, Rat, Chicken |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | The antibody was generated from a recombinant protein that includes amino acids in the N-terminal region of human Caveolin-1. |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purification | Antigen Affinity purification |
| Storage Buffer | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target | |
| Swiss Prot | Q03135 |
| Synonyms | caveolin1, vip21 |
| Background | Caveolins are the primary structural components of the plasma membrane microdomains, caveolae. Three members of the caveolin family (caveolin-1, -2, and -3) have been identified, and each has distinct expression patterns. Caveolins are involved in diverse biological functions, including vesicular trafficking, cholesterol homeostasis, cell adhesion and apoptosis. Caveolins can interact with various signaling molecules, including G-proteins, receptor tyrosine kinases, PKCs, and Src family kinases. Phosphorylation at Tyr-14 is essential for caveolin association with SH2 or PTB domain-containing adaptor proteins, while phosphorylation at Ser-80 regulates caveolin binding to the ER membrane and entry into the secretory pathway. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is Caveolin-1 (N-terminal region) Antibody (bs-70391R) validated for, and at what dilution?
bs-70391R is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Caveolin-1 (N-terminal region) Antibody (bs-70391R) react with?
Tested reactivity: Human, Mouse, Rat, Chicken.
How should bs-70391R be stored?
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. Storage buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
What immunogen was used to raise Caveolin-1 (N-terminal region) Antibody (bs-70391R)?
The antibody was generated from a recombinant protein that includes amino acids in the N-terminal region of human Caveolin-1.