CXCR4 (Ser-324/Ser-325), Phosphospecific Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bs-70442r-100uL |
| Product Name | CXCR4 (Ser-324/Ser-325), Phosphospecific Antibody |
| Applications |
WB
IHC
|
| Specificity | The antibody detects bands at 35, 42, 50, and 100 kDa* corresponding to various post-translationally modified forms of CXCR4 on SDS-PAGE immunoblots of human Jurkat and PC3 cells stimulated with calyculin A. |
| Reactivity | Human, Mouse, Rat, Chicken |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Phospho-CXCR4 (Ser-324/Ser-325) synthetic peptide (coupled to carrier protein) corresponds to amino acids surrounding Ser-324 and Ser-325 in the C-terminal region of human CXCR4. |
| Modification Site | S324/S325 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purification | Antigen Affinity purification |
| Storage Buffer | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target | |
| Swiss Prot | P61073 |
| Synonyms | FB22; HM89; LAP3; LCR1; NPYR; WHIM; CD184; LESTR; NPY3R; NPYRL; HSY3RR; NPYY3R; D2S201E; CXCR4 |
| Background | The chemokine receptor CXCR4 is a widely expressed G protein-coupled receptor required for development, hematopoiesis, organogenesis, and vascularization. In disease, CXCR4 has been implicated in WHIM syndrome, HIV, and cancers. Regulation of CXCR4 function occurs through phosphorylation at multiple sites in the C-terminal region. These sites have been shown to be phosphorylated after CXCL12 stimulation, and involve several kinases, such as PKC and GRK kinases. After CXCL12 stimulation of HEK293 cells, Ser-324 and Ser-325 become phosphorylated by PKC and GRK6, while Ser-330 and Ser-339 are phosphorylated by only GRK6. In human astroglia cells, Ser-324 and Ser-325 are rapidly phosphorylated in endogenous CXCR4, while Ser-330 was phosphorylated with slower kinetics. In addition, arrestin binding to CXCR4 is driven by this phosphorylation of far C-terminal residues. Thus, site-specific phosphorylation of CXCR4 may be regulated by multiple kinases lead to complex regulation of CXCR4 signaling. |
| Application Dilution | |
| WB | 1:300-5000 |
| IHC | |
Frequently asked questions
Which applications is CXCR4 (Ser-324/Ser-325), Phosphospecific Antibody (bs-70442R) validated for, and at what dilution?
bs-70442R is validated for WB (1:300-5000); IHC. These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CXCR4 (Ser-324/Ser-325), Phosphospecific Antibody (bs-70442R) react with?
Tested reactivity: Human, Mouse, Rat, Chicken.
How should bs-70442R be stored?
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. Storage buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
What immunogen was used to raise CXCR4 (Ser-324/Ser-325), Phosphospecific Antibody (bs-70442R)?
Phospho-CXCR4 (Ser-324/Ser-325) synthetic peptide (coupled to carrier protein) corresponds to amino acids surrounding Ser-324 and Ser-325 in the C-terminal region of human CXCR4.