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EphB1+EphB2 (Tyr594) Polyclonal Antibody, AbBy Fluor-750 Conjugated

Applications

  • WB
  • IF(IHC-P)

Reactivity

  • Human
  • Mouse
  • Rat
  • Bovine
Overview
Catalog # bs-7044r-bf750-100uL
Product Name EphB1+EphB2 (Tyr594) Polyclonal Antibody, AbBy Fluor-750 Conjugated
Applications
WB IF(IHC-P)
Reactivity Human, Mouse, Rat, Bovine
Specifications
Conjugation AbBy Fluor-750
Host Rabbit
Source KLH conjugated synthetic phosphopeptide derived from human EphB1+EphB2 around the phosphorylation site of Tyr594
Modification Site Tyr594
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 2047
Synonyms Eph receptor B1 + Eph receptor B2 phospho Y594:p-Eph receptor B1 + Eph receptor B2 phospho Y594Eph receptor B1+Eph receptor B2; EPH B1;EPH B2;Eph tyrosine kinase 2;Eph tyrosine kinase 3;EPHB1;EPHB2;Ephrin receptor EphB1;Ephrin receptor EphB2;Ephrin type B receptor 1;Ephrin type B receptor 2;EPHT 2;EPHT 3;ERK;Hek 5;Hek 6;Tyrosine protein kinase receptor EPH2;Tyrosine protein kinase receptor EPH3.
Background Ephrin receptors bind members of the ephrin ligand family. They are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. They make up the largest subgroup of the receptor tyrosine kinase (RTK) family. Eph receptor B1 (EphB1) and Eph receptor B2 (EphB2) are receptors for ephrin-B family members. Developing and adult neural tissue express nearly all of the Ephrin receptors and ephrin ligands. Ephrins and ephrin receptors also play a significant role in angiogenesis. EphB2 acts as a tumor suppressor.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200

Frequently asked questions

Which applications is EphB1+EphB2 (Tyr594) Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-7044R-BF750) validated for, and at what dilution?

bs-7044R-BF750 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does EphB1+EphB2 (Tyr594) Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-7044R-BF750) react with?

Tested reactivity: Human, Mouse, Rat, Bovine.

How should bs-7044R-BF750 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise EphB1+EphB2 (Tyr594) Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-7044R-BF750)?

KLH conjugated synthetic phosphopeptide derived from human EphB1+EphB2 around the phosphorylation site of Tyr594

What are the excitation and emission wavelengths of the AbBy Fluor-750 conjugate (bs-7044R-BF750)?

AbBy Fluor-750 on bs-7044R-BF750: excitation / emission 749nm/775nm.