EphB1+EphB2 (Tyr594) Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-7044r-cy55-100uL |
| Product Name | EphB1+EphB2 (Tyr594) Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Human, Mouse, Rat, Bovine |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human EphB1+EphB2 around the phosphorylation site of Tyr594 |
| Modification Site | Tyr594 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 2047 |
| Synonyms | Eph receptor B1 + Eph receptor B2 phospho Y594:p-Eph receptor B1 + Eph receptor B2 phospho Y594Eph receptor B1+Eph receptor B2; EPH B1;EPH B2;Eph tyrosine kinase 2;Eph tyrosine kinase 3;EPHB1;EPHB2;Ephrin receptor EphB1;Ephrin receptor EphB2;Ephrin type B receptor 1;Ephrin type B receptor 2;EPHT 2;EPHT 3;ERK;Hek 5;Hek 6;Tyrosine protein kinase receptor EPH2;Tyrosine protein kinase receptor EPH3. |
| Background | Ephrin receptors bind members of the ephrin ligand family. They are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. They make up the largest subgroup of the receptor tyrosine kinase (RTK) family. Eph receptor B1 (EphB1) and Eph receptor B2 (EphB2) are receptors for ephrin-B family members. Developing and adult neural tissue express nearly all of the Ephrin receptors and ephrin ligands. Ephrins and ephrin receptors also play a significant role in angiogenesis. EphB2 acts as a tumor suppressor. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is EphB1+EphB2 (Tyr594) Polyclonal Antibody, Cy5.5 Conjugated (BS-7044R-CY5-5) validated for, and at what dilution?
BS-7044R-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does EphB1+EphB2 (Tyr594) Polyclonal Antibody, Cy5.5 Conjugated (BS-7044R-CY5-5) react with?
Tested reactivity: Human, Mouse, Rat, Bovine.
How should BS-7044R-CY5-5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise EphB1+EphB2 (Tyr594) Polyclonal Antibody, Cy5.5 Conjugated (BS-7044R-CY5-5)?
KLH conjugated synthetic phosphopeptide derived from human EphB1+EphB2 around the phosphorylation site of Tyr594
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-7044R-CY5-5)?
Cy5.5 on BS-7044R-CY5-5: excitation / emission 675nm/694nm.