Fascin (Ser-39), Phosphospecific Antibody

Applications

Reactivity

Human
Mouse
Rat
Chicken

Overview
Catalog #	bs-70498r
Product Name	Fascin (Ser-39), Phosphospecific Antibody
Applications	WB
Specificity	This antibody was cross-adsorbed to a phospho-serine peptide before Affinity purification using phosho-fascin (Ser-39) peptide (without carrier). The antibody detects a 55 kDa* band corresponding to fascin on SDS-PAGE immunoblots of mouse C2C12 and human HeLa cells treated with Calyculin A.
Reactivity	Human, Mouse, Rat, Chicken
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Fascin (Ser-39) synthetic peptide (coupled to carrier protein) corresponds to amino acids surrounding serine 39 in human fascin.
Modification Site	Ser-39
Clonality	Polyclonal
Isotype	IgG
Purification	Antigen Affinity purification
Storage Buffer	PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Storage Condition	Storage at -20C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20C.
Target
Swiss Prot	Q16658
Synonyms	p55
Background	Fascin is an actin filament bundling protein localized to lamellipodia and filopodia where it has important roles in cell motility. Regulation of fascin occurs through PKC-mediated phosphorylation of Ser-39 in the F-actin binding site. Cell permeant peptides that block PKC phosphorylation of Ser-39 increase cell migration, while peptides that block fascin binding to F-actin alter lamellipodial morphology and cause aberrant cell motility. Studies using RNA interference of fascin show that fibroblasts have reduced number and abnormal morphology of filopodia, while Ser-39 phosphorylation status may determine filopodial frequency. In Drosophila neurons, fascin deficiency causes alterations in actin filaments and leads to abnormal morphology of developing neurons. Thus, fascin is a critical element of actin-based motility in various cell types.
Application Dilution
WB	1:300-5000