Girdin (Tyr-1764), Phosphospecific Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bs-70508r |
| Product Name | Girdin (Tyr-1764), Phosphospecific Antibody |
| Applications |
WB
|
| Specificity | The antibody was cross-absorbed to unphosphorylated Girdin (Tyr-1764) peptide before affinity purification using phospho-Girdin (Tyr-1764) peptide. This antibody detects a 250 kDa* protein on SDS-PAGE immunoblots of human A431 cells stimulated with EGF and Pervanadate. These reactivities are not observed after alkaline phosphatase treatment to dephosphorylate Girdin. This sequence has high homology to rat and mouse Girdin, and the site is not conserved in other Girdin family members. |
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Phospho-Girdin (Tyr-1764) synthetic peptide (coupled to carrier) corresponding to amino acid residues around tyrosine 1764 in human Girdin. |
| Modification Site | Tyr-1764 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purification | Antigen Affinity purification |
| Storage Buffer | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target | |
| Swiss Prot | Q3V6T2 |
| Synonyms | APE, Galpha, vesicle, GIV, Girders actin filament, HkRP1, GRDN, CCDC88A |
| Background | Girdin, a member of the CCDC88 (Hook related protein) family, is an actin binding protein involved with cell migration and maintaining cytoskeletal organization. Girdin has conserved domains at the N- and C-terminus that bind microtubules and actin, respectively. It enhances PI3-kinase dependent phosphorylation of proteins, most notably Akt. This same activity can contribute to tumor proliferation, invasion, and metastasis in breast, ovarian, prostate, and pancreatic tissues. Girdin is phosphorylated at three separate locations: Ser-1416, Ser-1674, and Tyr-1764. Ser-1416 is the primary Akt phosphorylation site, while Cyclin-dependent kinases interact with Girdin and phosphorylate Ser-1674. Multiple receptor tyrosine kinases can bind girdin and phosphorylate Tyr-1764. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is Girdin (Tyr-1764), Phosphospecific Antibody (bs-70508R) validated for, and at what dilution?
bs-70508R is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Girdin (Tyr-1764), Phosphospecific Antibody (bs-70508R) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-70508R be stored?
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. Storage buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
What immunogen was used to raise Girdin (Tyr-1764), Phosphospecific Antibody (bs-70508R)?
Phospho-Girdin (Tyr-1764) synthetic peptide (coupled to carrier) corresponding to amino acid residues around tyrosine 1764 in human Girdin.