DENTT Polyclonal Antibody

Formalin-fixed and paraffin embedded rat brain labeled with Anti DENTT Polyclonal Antibody, Unconjugated (bs-7051R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

L1 rat brain lysates L2 rat heart lysates probed with Anti DENTT Polyclonal Antibody, Unconjugated (bs-7051R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 76kD. Observed band size: 70kD

$Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (DENTT) polyclonal Antibody, Unconjugated (bs-7051R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.$

Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (DENTT) polyclonal Antibody, Unconjugated (bs-7051R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human

Predicted Reactivity

Mouse
Rat
Dog
Cow
Pig
Horse
Rabbit

Overview
Catalog #	bs-7051R
Product Name	DENTT Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Human
Predicted Reactivity	Mouse, Rat, Dog, Cow, Pig, Horse, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human DENTT
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	64061
Swiss Prot	Q9H2G4
Subcellular location	Cytoplasm, Nucleus
Synonyms	Testis-specific Y-encoded-like protein 2; TSPY-like protein 2; Cell division autoantigen 1; Cutaneous T-cell lymphoma-associated antigen se20-4; CTCL-associated antigen se20-4; Differentially-expressed nucleolar TGF-beta1 target protein; Nuclear protein of 79 kDa; NP79; TSPYL2; CDA1; DENTT; TSPX; HRIHFB2216
Background	Part of the CASK/TBR1/TSPYL2 transcriptional complex which modulates gene expression in response to neuronal synaptic activity, probably by facilitating nucleosome assembly. May inhibit cell proliferation by inducing p53-dependent CDKN1A expression.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200