Myosin light chain (phospho S20) Polyclonal Antibody, AbBy Fluor-750 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-7052R-BF750-100uL |
| Product Name | Myosin light chain (phospho S20) Polyclonal Antibody, AbBy Fluor-750 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat, Dog, Cow, Sheep, Pig, Rabbit |
| Specifications | |
| Conjugation | AbBy Fluor-750 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human Myosin Regulatory Light polypeptide 9 around the phosphorylation site of Ser20 |
| Modification Site | Ser20 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 10398 |
| Swiss Prot | P24844 |
| Subcellular location | Cytoplasm |
| Synonyms | LC20; MLC2; MRLC1; MYRL2; MLC-2C; Myosin regulatory light polypeptide 9; 20 kDa myosin light chain; Myosin RLC; Myosin regulatory light chain 2, smooth muscle isoform; Myosin regulatory light chain 9; Myosin regulatory light chain MRLC1; MYL9 |
| Background | Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Myosin light chain (phospho S20) Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-7052R-BF750) validated for, and at what dilution?
bs-7052R-BF750 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Myosin light chain (phospho S20) Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-7052R-BF750) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Cow, Sheep, Pig, Rabbit.
How should bs-7052R-BF750 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Myosin light chain (phospho S20) Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-7052R-BF750)?
KLH conjugated synthetic phosphopeptide derived from human Myosin Regulatory Light polypeptide 9 around the phosphorylation site of Ser20
What are the excitation and emission wavelengths of the AbBy Fluor-750 conjugate (bs-7052R-BF750)?
AbBy Fluor-750 on bs-7052R-BF750: excitation / emission 749nm/775nm.