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ELOVL5 Polyclonal Antibody, Cy5.5 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Rat

Predicted Reactivity

  • Mouse
  • Dog
  • Rabbit
Overview
Catalog # bs-7054R-Cy5.5-100uL
Product Name ELOVL5 Polyclonal Antibody, Cy5.5 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Rat
Predicted Reactivity Mouse, Dog, Rabbit
Specifications
Conjugation Cy5.5
Host Rabbit
Source KLH conjugated synthetic peptide derived from human ELOVL5
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 60481
Subcellular location Cytoplasm, Cell membrane
Synonyms 3 keto acyl CoA synthase ELOVL5; 3-keto acyl-CoA synthase Elovl5; dJ483K16.1; Elongation of very long chain fatty acids like 5; Elongation of very long chain fatty acids protein 5; ELOV5_HUMAN; ELOVL 5; ELOVL family member 5; ELOVL family member 5 elongation of long chain fatty acids; ELOVL family member 5, elongation of long chain fatty acids FEN1/Elo2, SUR4/Elo3-like, yeast ; ELOVL fatty acid elongase 5; ELOVL2; elovl5; Fatty acid elongase 1; HELO1; Homolog of yeast long chain polyunsaturated fatty acid elongation enzyme 2; OTTHUMP00000017867; RP3 483K16.1.
Background Condensing enzyme that catalyzes the synthesis of monounsaturated and of polyunsaturated very long chain fatty acids Acts specifically toward polyunsaturated acyl-CoA with the higher activity toward C18:3(n-6) acyl-CoA.Tissue specificity: Ubiquitous. Highly expressed in the adrenal gland and testis. Weekly expressed in prostate, lung and brain.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is ELOVL5 Polyclonal Antibody, Cy5.5 Conjugated (BS-7054R-CY5-5) validated for, and at what dilution?

BS-7054R-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does ELOVL5 Polyclonal Antibody, Cy5.5 Conjugated (BS-7054R-CY5-5) react with?

Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Dog, Rabbit.

How should BS-7054R-CY5-5 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise ELOVL5 Polyclonal Antibody, Cy5.5 Conjugated (BS-7054R-CY5-5)?

KLH conjugated synthetic peptide derived from human ELOVL5

What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-7054R-CY5-5)?

Cy5.5 on BS-7054R-CY5-5: excitation / emission 675nm/694nm.