Myosin IIA Heavy Chain (Ser-1803), Phosphospecific Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bs-70550r |
| Product Name | Myosin IIA Heavy Chain (Ser-1803), Phosphospecific Antibody |
| Applications |
WB
|
| Specificity | This antibody was cross-adsorbed to unphosphorylated Myosin IIA Heavy Chain (Ser-1803) peptide before affinity purification using phospho-Myosin IIA Heavy Chain (Ser-1803) peptide (without carrier). |
| Reactivity | Human, Mouse, Rat, Chicken |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Phospho-Myosin IIA Heavy Chain (Ser-1803) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding Ser-1803 in human myosin IIA heavy chain. |
| Modification Site | Ser-1803 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purification | Antigen Affinity purification |
| Storage Buffer | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target | |
| Swiss Prot | P35579 |
| Synonyms | NMHC-IIA, MYH9, myosin heavy chain |
| Background | Non-muscle myosin II is an actin-based motor protein essential to cell motility, division, migration, adhesion and polarity. This myosin forms a hexameric complex comprised of two heavy chains (NMHC-II), two essential light chains, and two regulatory light chains (RLC). In vertebrates, there are three NMHC-II isoforms (NMHC-IIA, NMHC-IIB, and NMHC-IIC), which exhibit distinct patterns of expression in cells and tissues. Regulation of NMHC-II activity occurs through RLC and HC phosphorylation. RLCs are phosphorylated at Thr-18 and Ser-19, which activates myosin II motor activity and promotes filament stability. By contrast, PKC phosphorylation of Ser-1/Ser-2 and Thr-9 in RLC may decrease activated myosin II interaction with actin. Several kinases phosphorylation NMHC-II isoforms directly. TRPM7 phosphorylates Thr-1800, Ser-1803, and Ser-1808, which reduces NMHC-IIA incorporation into the actin cytoskeleton. PKC phosphorylates NMHC-IIA on Ser-1916 and NMHC-IIB on multiple tailpiece serines leading to inhibition of filament assembly. Casein kinase II phosphorylates NMHC-IIA on Ser-1943 and increases disassembly of NMHC-IIA filaments. |
| Application Dilution | |
| WB | 1:300-5000 |