Phosphothreonine Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bs-70607r-100uL |
| Product Name | Phosphothreonine Antibody |
| Applications |
WB
IP
|
| Specificity | This antibody was cross-adsorbed to unphosphorylated peptide then Affinity purification using phospho-threonine peptide (without carrier). The antibody detects many serine or threonine phosphorylated proteins by western blot and ELISA. |
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Phosphothreonine synthetic peptides coupled to carrier protein. |
| Modification Site | pThr |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purification | Antigen Affinity purification |
| Storage Buffer | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target | |
| Swiss Prot | N/A |
| Synonyms | Phosphoser/thr mAb |
| Background | Phosphorylation of specific serine or threonine residues is an important post-translational modification for regulating the activity of most proteins. Stimulation of a variety of cell signaling pathways activates the receptor and non-receptor ser/thr kinases that mediate these protein modifications. Antibodies that can detect phosphoserine or phosphothreonine residues are excellent tools for characterizing changes in the post-translational state of a broad range of phosphorylated proteins. Immunoprecipitation of proteins of interest followed by detection of phosphoserine or phosphothreonine using anti-phosphoserine antibody is commonly used to correlate changes in phosphorylation state with alterations in protein activity |
| Application Dilution | |
| WB | 1:300-5000 |
| IP | 1-2ug |
Frequently asked questions
Which applications is Phosphothreonine Antibody (bs-70607R) validated for, and at what dilution?
bs-70607R is validated for WB (1:300-5000); IP (1-2ug). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phosphothreonine Antibody (bs-70607R) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-70607R be stored?
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. Storage buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
What immunogen was used to raise Phosphothreonine Antibody (bs-70607R)?
Phosphothreonine synthetic peptides coupled to carrier protein.