Help

NT5C1A Polyclonal Antibody, Cy3 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Predicted Reactivity

  • Human
  • Mouse
  • Rat
  • Dog
  • Cow
  • Pig
  • Horse
Overview
Catalog # bs-7060R-Cy3
Product Name NT5C1A Polyclonal Antibody, Cy3 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Predicted Reactivity Human, Mouse, Rat, Dog, Cow, Pig, Horse
Specifications
Conjugation Cy3
Host Rabbit
Source KLH conjugated synthetic peptide derived from human NT5C1A
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 84618
Subcellular location Cytoplasm
Synonyms NT5C1A dephosphorylates the 5' and 2'3'-phosphates of deoxyribonucleotides and has a broad substrate specicity. It helps to regulate adenosine levels in the heart during ischemia and hypoxia; 5NT1A_HUMAN.
Background NT5C1A dephosphorylates the 5' and 2'(3')-phosphates of deoxyribonucleotides and has a broad substrate specificity. It helps to regulate adenosine levels in the heart during ischemia and hypoxia.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is NT5C1A Polyclonal Antibody, Cy3 Conjugated (bs-7060R-Cy3) validated for, and at what dilution?

bs-7060R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

How should bs-7060R-Cy3 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise NT5C1A Polyclonal Antibody, Cy3 Conjugated (bs-7060R-Cy3)?

KLH conjugated synthetic peptide derived from human NT5C1A

What are the excitation and emission wavelengths of the Cy3 conjugate (bs-7060R-Cy3)?

Cy3 on bs-7060R-Cy3: excitation / emission 512,550nm/570,615nm.