phospho-PRKCA(S657/Y658) Polyclonal Antibody

Applications

Reactivity

Human

Overview
Catalog #	bs-70613r
Product Name	phospho-PRKCA(S657/Y658) Polyclonal Antibody
Applications	WB
Specificity	This antibody detects an 82kDa* protein corresponding to the molecular mass of phosphorylated PKC_ on SDS-PAGE immunoblots of neonatal rat brain lysate. Similar results were observed in human SKN-SH, endothelial, and HeLa cells, as well as rabbit spleen fibroblasts and rat pituitary cells.
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Phospho-PKC_ (Ser-657/Tyr-658) synthetic peptide corresponds to amino acid residues around serine 657 and tyrosine 658 of human PKC_.
Modification Site	S657/Y658
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Purification	Antigen Affinity purification
Storage Buffer	PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Storage Condition	Storage at -20C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20C.
Target
Swiss Prot	P17252
Synonyms	PKCalpha, PKCbeta, PKCgamma
Background	The Protein Kinase C (PKC) family of homologous serine/threonine protein kinases is involved in a number of processes such as growth, differentiation, and cytokine secretion. At least eleven isozymes have been described. PKC consists of a single polypeptide chain containing four conserved regions (C) and five variable regions (V). The N-terminal half interacts with PKC activators Ca2+, phospholipid, diacylglycerol, or phorbol ester, while the C-terminal half contains the catalytic domain. The conventional PKC subfamily (_, _1, _II, and _) is regulated by both Ca2+ and diacylglycerol. The PKC pathway represents a major signal transduction system that is activated following ligand-stimulation of transmembrane receptors by hormones, neurotransmitters and growth factors. The phosphorylation of multiple sites in conventional PKCs regulates their activity. In mast cells, FceRI stimulation leads to phosphorylation of tyrosine 658 and 662 of PKC_ and PKC_I respectively. This phosphorylation requires autophosphorylation of serine 657 and 661 in these respective kinases.
Application Dilution
WB	1:500-2000