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FAIM1 Polyclonal Antibody, Cy3 Conjugated

Applications

  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Zebrafish

Predicted Reactivity

  • Human
  • Mouse
  • Rat
  • Dog
  • Cow
  • Sheep
  • Pig
  • Horse
  • Chicken
  • Rabbit
Overview
Catalog # bs-7061R-Cy3
Product Name FAIM1 Polyclonal Antibody, Cy3 Conjugated
Applications
IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Zebrafish
Predicted Reactivity Human, Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit
Specifications
Conjugation Cy3
Host Rabbit
Source KLH conjugated synthetic peptide derived from human FAIM1
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 55179
Subcellular location Cytoplasm
Synonyms FAIM 1; FAIM; Fas apoptotic inhibitory molecule 1; Fas apoptotic inhibitory molecule; FAIM1_HUMAN.
Background FAIM1 (or Fas Apoptotic Inhibitory Molecule 1) is a recently discovered negative regulator of apoptosis. FAIM1 has no significant regions of homology to other gene products that modulate Fas killing. It plays a role as an inducible effector molecule that mediates Fas resistance produced by surface Ig engagement in B cells. Overexpression of FAIM1 diminishes sensitivity to Fas mediated apoptosis of B and non B cell lines. FAIM1 is highly evolutionarily conserved and is widely expressed in murine tissues, suggesting that FAIM1 plays an important role in cellular physiology.
Application Dilution
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is FAIM1 Polyclonal Antibody, Cy3 Conjugated (bs-7061R-Cy3) validated for, and at what dilution?

bs-7061R-Cy3 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does FAIM1 Polyclonal Antibody, Cy3 Conjugated (bs-7061R-Cy3) react with?

Tested reactivity: Zebrafish. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit.

How should bs-7061R-Cy3 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise FAIM1 Polyclonal Antibody, Cy3 Conjugated (bs-7061R-Cy3)?

KLH conjugated synthetic peptide derived from human FAIM1

What are the excitation and emission wavelengths of the Cy3 conjugate (bs-7061R-Cy3)?

Cy3 on bs-7061R-Cy3: excitation / emission 512,550nm/570,615nm.