| Overview |
| bs-70632r |
| PTP1B (N-terminal region) Antibody |
| WB |
| The antibody detects a 50 kDa* band corresponding to PTP1B in western blots of human A431, Jurkat, HeLa, and mouse SYF cells. This reactivity can be specifically blocked using PTP1B (a.a. 146-157) peptide (PX2355). This sequence has significant homology to the conserved site in rat and mouse PTP1B, and has homology to TC-PTP and MPTP, but not other protein tyrosine phosphatase family members. |
| Human, Mouse, Rat |
| Specifications |
| Unconjugated |
| Rabbit |
| PTP1B synthetic peptide (coupled to carrier protein) corresponds to amino acids from the N-terminal region of human PTP1B. |
| Polyclonal |
| #REF! |
| IgG |
| Antigen Affinity purification |
| PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage at -20C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20C. |
| Target |
| P18031 |
| PTPN1; TCPTP/PTPN2 |
| PTP1B is a nonreceptor type protein tyrosine phosphatase that has essential roles in insulin and leptin signaling, as well as important functions in growth factor and integrin signaling. The structure of PTP1B includes a conserved phosphatase domain, C-terminal hydrophobic residues for targeting to the cytoplasmic face of the endoplasmic reticulum, and proline-rich regions characteristic of SH3 domain binding motifs. PTP1B can interact with N-Cadherin and dephosphorylate _-catenin associated with cadherin complexes. PTP1B also interacts with Insulin and EGF receptors, and undergoes phosphorylation after receptor stimulation. Tyrosine phosphorylation at Tyr-66, Tyr-152, and Tyr-153 occurs after insulin receptor activation, and tyrosine phosphorylation of Tyr-152 may be required for interactions with N-Cadherin. In addition, Akt can phosphorylate Ser-50 and this phosphorylation can reduce PTP1B activity. |
| Application Dilution |
| WB |
1:300-5000 |
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