PTP1B (Tyr-152), Phosphospecific Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bs-70634r |
| Product Name | PTP1B (Tyr-152), Phosphospecific Antibody |
| Applications |
WB
|
| Specificity | This antibody was cross-adsorbed to a phospho-tyrosine peptide then Affinity purification with phospho-PTP1B (Tyr-152) peptide. The purified antibody detects a 50 kDa* band corresponding to PTP1B in western blots of pervanadate treated human A431 and Jurkat, as well as mouse SYF cells. |
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | PTP1B (Tyr-152) synthetic peptide corresponds to amino acids surrounding Tyr-152 in human PTP1B. |
| Modification Site | Tyr-152 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purification | Antigen Affinity purification |
| Storage Buffer | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target | |
| Swiss Prot | P18031 |
| Synonyms | PTPN1; TCPTP/PTPN2 |
| Background | PTP1B is a nonreceptor type protein tyrosine phosphatase that has essential roles in insulin and leptin signaling, as well as important functions in growth factor and integrin signaling. The structure of PTP1B includes a conserved phosphatase domain, C-terminal hydrophobic residues for targeting to the cytoplasmic face of the endoplasmic reticulum, and proline-rich regions characteristic of SH3 domain binding motifs. PTP1B can interact with N-Cadherin and dephosphorylate β-catenin associated with cadherin complexes. PTP1B also interacts with Insulin and EGF receptors, and undergoes phosphorylation after receptor stimulation. Tyrosine phosphorylation at Tyr-66, Tyr-152, and Tyr-153 occurs after insulin receptor activation, and tyrosine phosphorylation of Tyr-152 may be required for interactions with N-Cadherin. In addition, Akt can phosphorylate Ser-50 and this phosphorylation can reduce PTP1B activity. |
| Application Dilution | |
| WB | 1:300-5000 |