Semaphorin-3E (N-terminal region) Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bs-70653r |
| Product Name | Semaphorin-3E (N-terminal region) Antibody |
| Applications |
WB
|
| Specificity | The antibody detects 87 and 61 kDa bands corresponding to the full-length and furin-proccessed forms of Sema-3E in human PC-3 and rat PC12 cells. This sequence is well conserved in rat, mouse, and chicken Sema-3E, and has low homology to other semaphorin family members. |
| Reactivity | Human, Mouse, Rat, Chicken |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Sema-3E (N-terminal region) synthetic peptide (coupled to carrier protein) corresponds to amino acids in the N-terminal region of human Sema-3E. |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purification | Antigen Affinity purification |
| Storage Buffer | PBS + 0.05% NaN3 |
| Storage Condition | Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cy |
| Target | |
| Swiss Prot | O15041 |
| Background | The Semaphorin family of axon guidance molecules includes secreted, transmembrane, and GPI-anchored extracellular molecules that have been implicated in neuron development, vascular disease, and tumor progression. There are eight classes of semaphorin genes, all of which are characterized by a conserved 500 amino acid, cystine-rich Sema domain. Semaphorin 3E (Sema-3E) is a class III secreted semaphorin that binds Plexin D1 receptor, and has putative roles in axon guidance, angiogenesis, and cancers. Mutations in the Sema-3E gene are associated with CHARGE syndrome, a disorder that has nerve and cardiovascular abnormalities. The Sema-3E protein is expressed as full length (87 kDa) and furin-dependent processed forms (61 and 25 kDa). These forms are observed as monomers, as well as homo- and hetero-dimers. Sema-3E protein is frequently expressed in human cancer cell lines and in solid tumors from breast cancer patients. The expression of Sema-3E in mammary adenocarcinoma cells induces the ability to form experimental lung metastasis, and this activity requires furin-dependent production of the 61 kDa Sema-3E protein. |
| Application Dilution | |
| WB | 1:300-5000 |