Sphingosine Kinase 1 (Central region) Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bs-70674r-100uL |
| Product Name | Sphingosine Kinase 1 (Central region) Antibody |
| Applications |
WB
|
| Specificity | The antibody detects a 47 kDa* full-length recombinant human SK1 protein. In mouse, the antibody detects SK1 in isolated cardiac myocytes, but not in SK1-null mice (Tao, R. et al. (2007) Cardiovas. Res. 74:56.). This sequence has 4 amino acid differences from mouse and 5 from rat SK1, and is not homologous to sequences in SK2. This sequence has 4 amino acid differences from mouse and 5 from rat SK1, and is not homologous to sequences in SK2. |
| Reactivity | Human |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | SK1 synthetic peptide (coupled to carrier protein) corresponding to an unphosphorylated sequence of amino acids surrounding serine 225 in the central region of human SK1. |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purification | Antigen Affinity purification |
| Storage Buffer | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target | |
| Swiss Prot | Q9NYA1 |
| Synonyms | SK1, SPHK1, SPK1 |
| Background | Sphingolipids are metabolized into bioactive products that include ceramide, sphingosine, and sphingosine-1-phosphate (S1P). Sphingosine Kinase (SK) catalyzes the phosphorylation of the lipid sphingosine, creating S1P. S1P subsequently signals through cell surface G protein-coupled receptors, as well as intracellularly, to modulate cell proliferation, survival, motility and differentiation. Two isoforms of SK have been identified, SK1 and SK2. The mRNA for both of these isoforms is widely expressed with SK1 expression highest in brain, heart, kidney, thymus, spleen and lung, while SK2 is highest in kidney and liver. SKs can be activated through growth factor, G protein-coupled, and immunoglobulin receptor signalling. SK1 has been shown to mediate cell growth, prevention of apoptosis, and cellular transformation, and is upregulated in a variety of human tumors. Regulation of SK1 may occur through ERK mediated phosphorylation of Ser-225. This phosphorylation leads to increased activity and translocation to the plasma membrane. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is Sphingosine Kinase 1 (Central region) Antibody (bs-70674R) validated for, and at what dilution?
bs-70674R is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Sphingosine Kinase 1 (Central region) Antibody (bs-70674R) react with?
Tested reactivity: Human.
How should bs-70674R be stored?
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. Storage buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
What immunogen was used to raise Sphingosine Kinase 1 (Central region) Antibody (bs-70674R)?
SK1 synthetic peptide (coupled to carrier protein) corresponding to an unphosphorylated sequence of amino acids surrounding serine 225 in the central region of human SK1.