FKBP38 Polyclonal Antibody, Cy5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-7067R-Cy5 |
| Product Name | FKBP38 Polyclonal Antibody, Cy5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Dog, Cow, Horse, Chicken |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human FKBP38 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 23770 |
| Subcellular location | Cytoplasm |
| Synonyms | 38 kDa FK 506 binding protein homolog; 38 kDa FK506-binding protein; 38 kDa FKBP; 38kDa antibody FK506 binding protein 8; FK506 binding protein 8, 38kDa; FK506 binding protein, 38kDa; FK506-binding protein 8; FKBP 38; FKBP-38; FKBP-8; FKBP8; FKBP8_HUMAN; FKBPR38; hFKBP38; Peptidyl-prolyl cis-trans isomerase FKBP8; PPIase FKBP8; Rotamase; Sam11. |
| Background | Constitutively inactive PPiase, which becomes active when bound to calmodulin and calcium. Seems to act as a chaperone for BCL2, targets it to the mitochondria and modulates its phosphorylation state. The BCL2/FKBP8/calmodulin/calcium complex probably interferes with the binding of BCL2 to its targets. The active form of FKBP8 may therefore play a role in the regulation of apoptosis. Homomultimers or heteromultimers (Potential). Forms heterodimer with calmodulin. When activated by calmodulin and calcium, interacts with the BH4 domain of BCL2 and weakly with BCLX isoform Bcl-X(L). Does not bind and inhibit calcineurin. Interacts with HCV NS5A. Interacts with ZFYVE27; may negatively regulate ZFYVE27 phosphorylation.Tissue specificity: Widely expressed. Highest levels seen in the brain. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is FKBP38 Polyclonal Antibody, Cy5 Conjugated (bs-7067R-Cy5) validated for, and at what dilution?
bs-7067R-Cy5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does FKBP38 Polyclonal Antibody, Cy5 Conjugated (bs-7067R-Cy5) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Cow, Horse, Chicken.
How should bs-7067R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise FKBP38 Polyclonal Antibody, Cy5 Conjugated (bs-7067R-Cy5)?
KLH conjugated synthetic peptide derived from human FKBP38
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-7067R-Cy5)?
Cy5 on bs-7067R-Cy5: excitation / emission 625,650nm/670nm.