NME5 Polyclonal Antibody, Cy3 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-7073R-Cy3 |
| Product Name | NME5 Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Sheep, Horse, Rabbit |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human NME5/IPIA beta |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 8382 |
| Subcellular location | Nucleus |
| Synonyms | Inhibitor of p53 induced apoptosis beta; IPIA beta; NDK-H 5; NDP kinase homolog 5; NM23 H5; Nm23-M5; NM23H5; Non-metastatic cells 5, protein expressed in nucleoside-diphosphate kinase; Nonmetastatic cells 5, protein expressed in; Nonmetastatic protein 23, homolog 5; Nucleoside diphosphate kinase homolog 5; RSPH23; Testis specic nm23 homolog; NDK5_HUMAN. |
| Background | NME5 does not seem to have NDK kinase activity but confers protection from cell death by Bax and alters the cellular levels of several antioxidant enzymes including Gpx5. It may play a role in spermiogenesis by increasing the ability of late-stage spermatids to eliminate reactive oxygen species. NME5 is specifically expressed in testis germinal cells. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is NME5 Polyclonal Antibody, Cy3 Conjugated (bs-7073R-Cy3) validated for, and at what dilution?
bs-7073R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-7073R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise NME5 Polyclonal Antibody, Cy3 Conjugated (bs-7073R-Cy3)?
KLH conjugated synthetic peptide derived from human NME5/IPIA beta
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-7073R-Cy3)?
Cy3 on bs-7073R-Cy3: excitation / emission 512,550nm/570,615nm.