Nucleolar protein 3 Polyclonal Antibody, HRP Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-7080R-HRP |
| Product Name | Nucleolar protein 3 Polyclonal Antibody, HRP Conjugated |
| Applications |
ELISA
IHC-P
IHC-F
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Dog, Cow, Rabbit |
| Specifications | |
| Conjugation | HRP |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from mouse Nucleolar protein 3/Apoptosis repressor with CARD |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 8996 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | Apoptosis repressor with CARD; ARC; Muscle enriched cytoplasmic protein; MYC; Myp; NOP; Nop30; Nucleolar protein 3 apoptosis repressor with CARD domain; Nucleolar protein 3; Nucleolar protein of 30 kDa; NOL3_MOUSE . |
| Background | Apoptosis is regulated by death domain (DD) and/or caspase recruitment domain (CARD)bcontaining molecules and a caspase family of proteases. CARD containing cell death regulators include RAIDD, RICK, BCL10, Apaf 1, caspase 9 and caspase 2. Apoptosis repressor with CARD is a CARD domain containing protein that interacts with caspase 2 and 8 to inhibit enzymatic activity of caspase 8. Apoptosis repressor with CARD suppresses apoptosis induced by cell death adapters FADD and TRADD and by cell death receptors Fas, TNFR 1, and DR3. The mRNA of Apoptosis repressor with CARD is primarily expressed in skeletal muscle and cardiac tissue. The nuclear isoform (1/Nop30) may be involved in RNA splicing and the cytoplasmic isoform (2/Myp) may inhibit apoptosis. |
| Application Dilution | |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
Frequently asked questions
Which applications is Nucleolar protein 3 Polyclonal Antibody, HRP Conjugated (bs-7080R-HRP) validated for, and at what dilution?
bs-7080R-HRP is validated for ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Nucleolar protein 3 Polyclonal Antibody, HRP Conjugated (bs-7080R-HRP) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Rabbit.
How should bs-7080R-HRP be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Nucleolar protein 3 Polyclonal Antibody, HRP Conjugated (bs-7080R-HRP)?
KLH conjugated synthetic peptide derived from mouse Nucleolar protein 3/Apoptosis repressor with CARD