CARD15/NOD2 Polyclonal Antibody, AbBy Fluor-647 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-7084R-BF647-100uL |
| Product Name | CARD15/NOD2 Polyclonal Antibody, AbBy Fluor-647 Conjugated |
| Applications |
WB
FCM
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Cow, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | AbBy Fluor-647 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CARD15 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 64127 |
| Swiss Prot | Q9HC29 |
| Subcellular location | Cytoplasm |
| Synonyms | ACUG; Arthrocutaneouveal granulomatosis; BLAU; CARD15; Caspase recruitment domain protein 15; Caspase recruitment domain-containing protein 15; IBD1; Inflammatory bowel disease protein 1; LRR containing protein; LRR-containing protein; Nod2; NOD2 protein; NOD2_HUMAN; NOD2B; nucleotide binding oligomerization domain 2; nucleotide-binding oligomerization domain 2; Nucleotide-binding oligomerization domain-containing protein 2; PSORAS1. |
| Background | Defects in NOD2 are the cause of sarcoidosis early-onset (EOS) . EOS is a form of sarcoidosis manifesting in children younger than 4 years of age. Sarcoidosis is an idiopathic, systemic, inflammatory disease characterized by the formation of immune granulomas in involved organs. Granulomas predominantly invade the lungs and the lymphatic system, but also skin, liver, spleen, eyes and other organs may be involved. Early-onset sarcoidosis is quite rare and has a distinct triad of skin, joint and eye disorders, without apparent pulmonary involvement. Compared with an asymptomatic and sometimes naturally disappearing course of the disease in older children, early-onset sarcoidosis is progressive and in many cases causes severe complications, such as blindness, joint destruction and visceral involvement. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is CARD15/NOD2 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-7084R-BF647) validated for, and at what dilution?
bs-7084R-BF647 is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CARD15/NOD2 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-7084R-BF647) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Pig, Horse, Rabbit.
How should bs-7084R-BF647 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CARD15/NOD2 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-7084R-BF647)?
KLH conjugated synthetic peptide derived from human CARD15
What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bs-7084R-BF647)?
AbBy Fluor-647 on bs-7084R-BF647: excitation / emission 650nm/665nm.