CARD6 Polyclonal Antibody, Cy5 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-7087R-Cy5 |
| Product Name | CARD6 Polyclonal Antibody, Cy5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Rat, Dog, Horse, Rabbit |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CARD6 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 84674 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | CARD 6; CARD containing inhibitor of Nod1 and Cardiak-induced NF kB activation; CARD6; CARD6_HUMAN; Caspase recruitment domain family, member 6; Caspase recruitment domain protein 6; Caspase recruitment domain-containing protein 6; CINCIN1; D730008L15. |
| Background | This gene encodes a protein that contains a caspase recruitment domain (CARD), an antiparallel six-helical bundle that mediates homotypic protein-protein interactions. The encoded protein is a microtubule-associated protein that has been shown to interact with receptor-interacting protein kinases and positively modulate signal transduction pathways converging on activation of the inducible transcription factor NF-kB. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is CARD6 Polyclonal Antibody, Cy5 Conjugated (bs-7087R-Cy5) validated for, and at what dilution?
bs-7087R-Cy5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-7087R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CARD6 Polyclonal Antibody, Cy5 Conjugated (bs-7087R-Cy5)?
KLH conjugated synthetic peptide derived from human CARD6
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-7087R-Cy5)?
Cy5 on bs-7087R-Cy5: excitation / emission 625,650nm/670nm.