CARD8 Polyclonal Antibody
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-7088R |
| Product Name | CARD8 Polyclonal Antibody |
| Applications |
WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CARD8 |
| Immunogen Range | 221-320/431 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 22900 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | Apoptotic protein NDPP 1; Apoptotic protein NDPP1; CARD 8; CARD inhibitor of NF kappa B activating ligand; CARD inhibitor of NF kappaB activating ligand; CARD inhibitor of NF kappaB activating ligands; CARD-inhibitor of NF-kappa-B-activating ligand; CARD8; CARD8_HUMAN; CARDINAL; Caspase recruitment domain containing protein 8; Caspase recruitment domain family member 8; Caspase recruitment domain protein 8; Caspase recruitment domain-containing protein 8; DACAR; NDPP 1; NDPP1; TUCAN; Tumor up regulated CARD containing antagonist of CASP9; Tumor up regulated CARD containing antagonist of caspase 9; Tumor up-regulated CARD-containing antagonist of CASP9; Tumor upregulated CARD containing antagonist of CASP9. |
| Background | Inhibits NF-kappa-B activation. May participate in a regulatory mechanism that coordinates cellular responses controlled by NF-kappa-B transcription factor. May be a component of the inflammasome, a protein complex which also includes PYCARD, NALP2 and CASP1 and whose function would be the activation of proinflammatory caspases. |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is CARD8 Polyclonal Antibody (bs-7088R) validated for, and at what dilution?
bs-7088R is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-7088R be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CARD8 Polyclonal Antibody (bs-7088R)?
KLH conjugated synthetic peptide derived from human CARD8 Immunogen range: 221-320/431.