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CARD9 Polyclonal Antibody

Applications

  • WB
  • ELISA
  • IHC-P
  • IHC-F
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Predicted Reactivity

  • Human
  • Dog
  • Horse
Overview
Catalog # bs-7089R
Product Name CARD9 Polyclonal Antibody
Applications
WB ELISA IHC-P IHC-F IF(IHC-P) IF(IHC-F) IF(ICC)
Predicted Reactivity Human, Dog, Horse
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human CARD9
Immunogen Range 101-200/536
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 64170
Subcellular location Cytoplasm, Nucleus, Secreted, Cell membrane, Extracellular matrix
Synonyms CARD9;CARD9_HUMAN;Caspase recruitment domain family member 9;Caspase recruitment domain-containing protein 9;hCARD9.
Background Activates NF-kappa-B via BCL10.Tissue specificity; Highly expressed in spleen. Also detected in liver, placenta, lung, peripheral blood leukocytes and in brain.
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is CARD9 Polyclonal Antibody (bs-7089R) validated for, and at what dilution?

bs-7089R is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

How should bs-7089R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise CARD9 Polyclonal Antibody (bs-7089R)?

KLH conjugated synthetic peptide derived from human CARD9 Immunogen range: 101-200/536.