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TP53I11 Polyclonal Antibody, Cy5 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Predicted Reactivity

  • Human
  • Mouse
  • Rat
  • Dog
  • Cow
  • Sheep
  • Pig
  • Horse
  • Rabbit
Overview
Catalog # bs-7091R-Cy5
Product Name TP53I11 Polyclonal Antibody, Cy5 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Predicted Reactivity Human, Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Rabbit
Specifications
Conjugation Cy5
Host Rabbit
Source KLH conjugated synthetic peptide derived from human TP53I11
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 9537
Subcellular location Cell membrane
Synonyms p53 induced gene 11 protein; p53 induced protein; PIG 11; PIG11; Tumor protein p53 inducible protein 11; P5I11_HUMAN.
Background TP53I11 (Tumor protein p53 inducible protein 11), also referred to as PIG11 (p53-induced gene 11), is an early transcriptional target of tumor suppressor p53. It is up-regulated in the induction of apoptosis or cell growth inhibition by multiple chemopreventive agents. However, its biological role remains unclear.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is TP53I11 Polyclonal Antibody, Cy5 Conjugated (bs-7091R-Cy5) validated for, and at what dilution?

bs-7091R-Cy5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

How should bs-7091R-Cy5 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise TP53I11 Polyclonal Antibody, Cy5 Conjugated (bs-7091R-Cy5)?

KLH conjugated synthetic peptide derived from human TP53I11

What are the excitation and emission wavelengths of the Cy5 conjugate (bs-7091R-Cy5)?

Cy5 on bs-7091R-Cy5: excitation / emission 625,650nm/670nm.