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CLIC4 Polyclonal Antibody, RBITC Conjugated

Applications

  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Predicted Reactivity

  • Human
  • Mouse
  • Rat
  • Dog
  • Cow
  • Sheep
  • Pig
  • Horse
  • Chicken
  • Rabbit
Overview
Catalog # bs-7098R-RBITC-100uL
Product Name CLIC4 Polyclonal Antibody, RBITC Conjugated
Applications
IF(IHC-P) IF(IHC-F) IF(ICC)
Predicted Reactivity Human, Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit
Specifications
Conjugation RBITC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human CLIC4
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 25932
Subcellular location Cytoplasm, Nucleus, Cell membrane
Synonyms MC3S5; Chloride intracellular channel 4; Chloride intracellular channel 4 mitochondrial; Chloride intracellular channel 4 like; Chloride intracellular channel protein 4; Clic4; CLIC4_HUMAN; CLIC4L; DKFZP566G223; FLJ38640; H1 antibody HUH1; Intracellular chloride ion channel protein p64H1; mtCLIC; p64H1.
Background Can insert into membranes and form poorly selective ion channels that may also transport chloride ions. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. Promotes cell-surface expression of HRH3. Has alternate cellular functions like a potential role in angiogenesis or in maintaining apical-basolateral membrane polarity during mitosis and cytokinesis. Could also promote endothelial cell proliferation and regulate endothelial morphogenesis.Tissue specificity: Detected in epithelial cells from colon, esophagus and kidney (at protein level). Expression is prominent in heart, kidney, placenta and skeletal muscle.
Application Dilution
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is CLIC4 Polyclonal Antibody, RBITC Conjugated (bs-7098R-RBITC) validated for, and at what dilution?

bs-7098R-RBITC is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

How should bs-7098R-RBITC be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise CLIC4 Polyclonal Antibody, RBITC Conjugated (bs-7098R-RBITC)?

KLH conjugated synthetic peptide derived from human CLIC4

What are the excitation and emission wavelengths of the RBITC conjugate (bs-7098R-RBITC)?

RBITC on bs-7098R-RBITC: excitation / emission 540nm/625nm.

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