CCDC139 Polyclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bs-7102R |
| Product Name | CCDC139 Polyclonal Antibody |
| Applications |
WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human PUS10/CCDC139 |
| Immunogen Range | 101-200/529 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | CCDC139; Coiled coil domain containing protein 139; DOBI; FLJ32312; MGC126729; Putative tRNA pseudouridine synthase Pus10; tRNA pseudouridine 55 synthase; tRNA uridine isomerase; PUS10_HUMAN. |
| Background | Pseudouridination is the isomerization of uridine to pseudouridine. It is the most common posttranscriptional nucleotide modification found in RNA. It is essential for biologic functions such as spliceosome biogenesis. Pseudouridylate synthases, such as PUS10, catalyze pseudouridination of structural RNAs, including transfer, ribosomal, and splicing RNAs. These enzymes also act as RNA chaperones which facilitate the correct folding and assembly of tRNAs. |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is CCDC139 Polyclonal Antibody (bs-7102R) validated for, and at what dilution?
bs-7102R is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CCDC139 Polyclonal Antibody (bs-7102R) react with?
Tested reactivity: Human.
How should bs-7102R be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CCDC139 Polyclonal Antibody (bs-7102R)?
KLH conjugated synthetic peptide derived from human PUS10/CCDC139 Immunogen range: 101-200/529.