THAP1 Polyclonal Antibody

$Lane 1: Mouse Muscle tissue lysates; Lane 2: Mouse Kidney tissue lysates; Lane 3: Mouse Liver tissue lysates; Lane 4: Mouse Thymus tissue lysates ; Lane 5: Rat Heart tissue lysates ; Lane 6: Rat Muscle tissue lysates ; Lane 7: Rat Kidney tissue lysates ; Lane 8: Rat Liver tissue lysates ; Lane 9: Rat Thymus tissue lysates ; Lane 10: Human Molt-4 cell lysates ; Lane 11: Human Huvec cell lysates ; Lane 12: Human U251 cell lysates probed with THAP1 Polyclonal Antibody, Unconjugated (bs-7103R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Muscle tissue lysates; Lane 2: Mouse Kidney tissue lysates; Lane 3: Mouse Liver tissue lysates; Lane 4: Mouse Thymus tissue lysates ; Lane 5: Rat Heart tissue lysates ; Lane 6: Rat Muscle tissue lysates ; Lane 7: Rat Kidney tissue lysates ; Lane 8: Rat Liver tissue lysates ; Lane 9: Rat Thymus tissue lysates ; Lane 10: Human Molt-4 cell lysates ; Lane 11: Human Huvec cell lysates ; Lane 12: Human U251 cell lysates probed with THAP1 Polyclonal Antibody, Unconjugated (bs-7103R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Dog
Cow
Sheep
Chicken
Rabbit

Overview
Catalog #	bs-7103R
Product Name	THAP1 Polyclonal Antibody
Applications	ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Dog, Cow, Sheep, Chicken, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human THAP1
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	55145
Subcellular location	Nucleus
Synonyms	Nuclear proapoptotic factor; THAP 1; THAP domain containing apoptosis associated protein 1; THAP domain containing protein 1; THAP domain protein 1; THAP1_HUMAN.
Background	THAP1 contains a THAP domain, a zinc-dependent DNA-binding domain. It colocalizes with the apoptosis response protein PAWR/PAR-4 in promyelocytic leukemia (PML) nuclear bodies and is a pro-apoptopic protein that potentiates both serum-withdrawal and TNF-induced apoptosis. It is a physiologic regulator of endothelial cell proliferation and cell-cycle progression, two essential processes for angiogenesis.
Application Dilution
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200