FEM 1B Polyclonal Antibody, Cy5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-7113R-Cy5 |
| Product Name | FEM 1B Polyclonal Antibody, Cy5 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse, Cow, Sheep, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human FEM 1B |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 10116 |
| Swiss Prot | Q9UK73 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | F1AA; F1A-ALPHA; FEM1-beta; Protein fem-1 homolog B; FEM1b; Fem-1-like death receptor-binding protein alpha; Fem-1-like in apoptotic pathway protein alpha; KIAA0396 |
| Background | Component of an E3 ubiquitin-protein ligase complex, in which it may act as a substrate recognition subunit. Involved in apoptosis by acting as a death receptor-associated protein that mediates apoptosis. Also involved in glucose homeostasis in pancreatic islet. Functions as an adapter/mediator in replication stress-induced signaling that leads to the activation of CHEK1. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is FEM 1B Polyclonal Antibody, Cy5 Conjugated (bs-7113R-Cy5) validated for, and at what dilution?
bs-7113R-Cy5 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does FEM 1B Polyclonal Antibody, Cy5 Conjugated (bs-7113R-Cy5) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Cow, Sheep, Horse, Chicken, Rabbit.
How should bs-7113R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise FEM 1B Polyclonal Antibody, Cy5 Conjugated (bs-7113R-Cy5)?
KLH conjugated synthetic peptide derived from human FEM 1B
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-7113R-Cy5)?
Cy5 on bs-7113R-Cy5: excitation / emission 625,650nm/670nm.