Rad23 Polyclonal Antibody, PerCP-Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-7121r-percp-cy7-100uL |
| Product Name | Rad23 Polyclonal Antibody, PerCP-Cy7 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse |
| Predicted Reactivity | yeast |
| Specifications | |
| Conjugation | PerCP-Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from Saccharomyces cerevisiae Rad23 |
| Immunogen Range | 51-150/398 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 856674 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | DNA repair protein RAD23; UV excision repair protein RAD23; RAD23_YEAST; YEL037C; SYGP-ORF29. |
| Background | The RAD23 gene of Saccharomyces cerevisiae is required for excision-repair of UV damaged DNA (1). RAD23 resembles the other DNA repair genes, RAD2, RAD6, RAD7, RAD18, and RAD54, all of which also exhibit increased transcription in response to DNA damage and during meiosis (2). RAD23 encodes a nuclear protein containing a ubiquitin-like domain required for biological function (3). RAD23 bears a ubiquitin-like domain at its amino terminus and this ubiquitin-like domain affects protein function in a nonproteolytic manner (3). Rad23 is a highly conserved protein involved in nucleotide excision repair (NER) that associates with the proteasome via its amino-terminus (4). Its carboxy-terminal ubiquitin-associated domain is evolutionarily conserved from yeast to humans (4). In addition to a role in DNA repair events in yeast, several lines of evidence indicate that the Rad23 protein may regulate the activity of the 26 S proteasome (5). |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Rad23 Polyclonal Antibody, PerCP-Cy7 Conjugated (bs-7121R-PerCP-Cy7) validated for, and at what dilution?
bs-7121R-PerCP-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Rad23 Polyclonal Antibody, PerCP-Cy7 Conjugated (bs-7121R-PerCP-Cy7) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): yeast.
How should bs-7121R-PerCP-Cy7 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Rad23 Polyclonal Antibody, PerCP-Cy7 Conjugated (bs-7121R-PerCP-Cy7)?
KLH conjugated synthetic peptide derived from Saccharomyces cerevisiae Rad23 Immunogen range: 51-150/398.
What are the excitation and emission wavelengths of the PerCP-Cy7 conjugate (bs-7121R-PerCP-Cy7)?
PerCP-Cy7 on bs-7121R-PerCP-Cy7: excitation / emission 482nm/780nm.