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NMDA epsilon 1 Polyclonal Antibody, AbBy Fluor-647 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human

Predicted Reactivity

  • Mouse
  • Rat
  • Dog
  • Cow
  • Sheep
Overview
Catalog # bs-7124R-BF647-100uL
Product Name NMDA epsilon 1 Polyclonal Antibody, AbBy Fluor-647 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human
Predicted Reactivity Mouse, Rat, Dog, Cow, Sheep
Specifications
Conjugation AbBy Fluor-647
Host Rabbit
Source KLH conjugated synthetic peptide derived from human NMDA epsilon 1
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 2902
Subcellular location Cell membrane
Synonyms N-Methyl-d-Asprtate receptor epsilon 1; Glutamate Receptor Ionotropic N Methyl D Aspartate epsilon-1; N Methly D Aspartate Receptor Channel Subunit Epsilon 1; NMDZ1_HUMAN.
Background Glutamate receptors mediate most excitatory neurotransmission in the brain and play an important role in neural plasticity, neural development and neurodegeneration. Ionotropic glutamate receptors are categorized into NMDA receptors and kainate/AMPA receptors, both of which contain glutamate-gated, cation-specific ion channels. Kainate/AMPA receptors are co-localized with NMDA receptors in many synapses and consist of seven structurally related subunits designated GluR-1 to -7. The kainate/AMPA receptors are primarily responsible for fast excitatory neurotransmission by glutamate, whereas the NMDA receptors exhibit slow kinetics of Ca2+ ions and a high permeability for Ca2+ ions.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is NMDA epsilon 1 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-7124R-BF647) validated for, and at what dilution?

bs-7124R-BF647 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does NMDA epsilon 1 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-7124R-BF647) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Sheep.

How should bs-7124R-BF647 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise NMDA epsilon 1 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-7124R-BF647)?

KLH conjugated synthetic peptide derived from human NMDA epsilon 1

What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bs-7124R-BF647)?

AbBy Fluor-647 on bs-7124R-BF647: excitation / emission 650nm/665nm.

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