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Mitoferrin 2/SLC25A28 Polyclonal Antibody, AbBy Fluor-350 Conjugated

Applications

  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Mouse
  • Rat

Predicted Reactivity

  • Human
  • Dog
  • Cow
  • Sheep
  • Pig
Overview
Catalog # bs-7157R-BF350-100uL
Product Name Mitoferrin 2/SLC25A28 Polyclonal Antibody, AbBy Fluor-350 Conjugated
Applications
IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Mouse, Rat
Predicted Reactivity Human, Dog, Cow, Sheep, Pig
Specifications
Conjugation AbBy Fluor-350
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Mitoferrin 2
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 81894
Swiss Prot Q96A46
Subcellular location Cytoplasm
Synonyms MFRN2; Mitochondrial iron transporter 2; Mitochondrial RNA splicing protein 3/4; Mitoferrin 2; Mitoferrin2; Mitoferrin-2; MRS3/4; MRS4L; NPD016; Putative mitochondrial solute carrier; MFRN2_HUMAN; SLC25A28.
Background Mitoferrin 2, also known as MRS3/4 (mitochondrial RNA-splicing protein 3/4 homolog), mitochondrial iron transporter 2, NPD016, MRS4L or SLC25A28 (solute carrier family 25 member 28), is a 364 amino acid multi-pass membrane protein of the mitochondrial inner membrane that mediates iron uptake. Mitoferrin 2 is thought to play a role in heme synthesis of hemoproteins and iron-sulfur cluster assembly. Ubiquitously expressed, Mitoferrin 2 is found at high levels in skeletal muscle, heart, placenta, kidney, lung, liver, brain and pancreas. Mitoferrin 2 is a member of the mitochondrial carrier family and undergoes alternative splicing events to produce four isoforms. Mitoferrin 2 contains three solcar repeats and is encoded by a gene that maps to human chromosome 10q24.2.
Application Dilution
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is Mitoferrin 2/SLC25A28 Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-7157R-BF350) validated for, and at what dilution?

bs-7157R-BF350 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Mitoferrin 2/SLC25A28 Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-7157R-BF350) react with?

Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Cow, Sheep, Pig.

How should bs-7157R-BF350 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Mitoferrin 2/SLC25A28 Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-7157R-BF350)?

KLH conjugated synthetic peptide derived from human Mitoferrin 2

What are the excitation and emission wavelengths of the AbBy Fluor-350 conjugate (bs-7157R-BF350)?

AbBy Fluor-350 on bs-7157R-BF350: excitation / emission 346nm/442nm.

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