Sumo2+3 Polyclonal Antibody, FITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-7338R-FITC |
| Product Name | Sumo2+3 Polyclonal Antibody, FITC Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Cow, Horse, Chicken, Rabbit, Zebrafish |
| Specifications | |
| Conjugation | FITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Sumo 2+3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Nucleus |
| Synonyms | HSMT3; MGC117191; OTTHUMP00000115275; OTTHUMP00000115276; OTTHUMP00000115277; Sentrin 2; Small ubiquitin like modier 2; Small ubiquitin like modier protein 3; Small ubiquitin related modier 2; Small ubiquitin related modier 3; SMT3 A; SMT3 B; SMT3 H1; SMT3 H2; SMT3 homolog 1; SMT3 homolog 2; SMT3 homolog; SMT3 suppressor of m two 3 homolog 1; SMT3 suppressor of m two 3 homolog 2 S. cerevisiae; SMT3 suppressor of m two 3 homolog 2; SMT3 suppressor of m two 3 homolog 3 S. cerevisiae; SMT3 suppressor of m two 3 homolog 3; SMT3A; SMT3B; SMT3H1; SMT3H2; Sumo 2; Sumo 3; Sumo2; Sumo3; Suppressor of m two 3 homolog 2; Suppressor of m two 3 homolog 3; Ubiquitin like protein SMT3A; Ubiquitin like protein SMT3B; SUMO2_HUMAN; SUMO3_HUMAN. |
| Background | The small ubiquitin-related modifier (SUMO) proteins, which include SUMO-1, SUMO-2 and SUMO-3, belong to the ubiquitin-like protein family. Like ubiquitin, the SUMO proteins are synthesized as precursor proteins that undergo processing before conjugation to target proteins. Also, both utilize the E1, E2, and E3 cascade enzymes for conjugation. However, SUMO and ubiquitin differ with respect to targeting. Ubiquitination predominantly targets proteins for degradation, whereas sumoylation targets proteins to a variety of cellular processing, including nuclear transport, transcriptional regulation, apoptosis and protein stability. The unconjugated SUMO-1, SUMO-2 and SUMO-3 proteins localize to the nuclear membrane, nuclear bodies and cytoplasm, respectively. SUMO-1 utilizes Ubc9 for conjugation to several target proteins, which include IkBa, MDM2, p53, PML and Ran GAP1. SUMO-2 and SUMO-3 contribute to a greater percentage of protein modification than does SUMO-1, and unlike SUMO-1, they can form polymeric chains. In addition, SUMO-3 regulates b-Amyloid generation and may be critical in the onset or progression of Alzheimer?s disease. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Sumo2+3 Polyclonal Antibody, FITC Conjugated (bs-7338R-FITC) validated for, and at what dilution?
bs-7338R-FITC is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Sumo2+3 Polyclonal Antibody, FITC Conjugated (bs-7338R-FITC) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Cow, Horse, Chicken, Rabbit, Zebrafish.
How should bs-7338R-FITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Sumo2+3 Polyclonal Antibody, FITC Conjugated (bs-7338R-FITC)?
KLH conjugated synthetic peptide derived from human Sumo 2+3
What are the excitation and emission wavelengths of the FITC conjugate (bs-7338R-FITC)?
FITC on bs-7338R-FITC: excitation / emission 494nm/518nm.