OAS3 Polyclonal Antibody, Cy5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-7344R-Cy5 |
| Product Name | OAS3 Polyclonal Antibody, Cy5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human OAS3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4940 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | 2-5''oligoA synthase 3; 2 5' oligoA synthetase 3; 2 5'LIGO; 2 5A synthetase 3; 2''-5''-oligoadenylate synthase 3; 2-5A synthase 3; MGC133260; OAS3 2' 5' oligoadenylate synthetase 3, 100kDa; OAS3; OAS3_HUMAN; p100; p100 OAS; p100OAS. |
| Background | The 2'- 5'- oligoadenylate synthetase (OAS) family is comprised of four members: OAS1, OAS2, OAS3 and OASL. These proteins are induced by interferons and function to convert ATP into 2'- 5'- linked oligomers of adenosine in the presence of double-stranded RNA and magnesium ions. Copper, iron and zinc ions strongly inhibit the OAS enzymatic activity, while manganese ions can replace magnesium ions as an activator. The OAS family plays a significant role in the inhibition of cellular protein synthesis, apoptosis and growth, and its members are important factors in viral infection resistance. OAS3, also referred to as p100, contains three adjacent OAS1-like domains and maps to the human chromosome 12q24.2 |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is OAS3 Polyclonal Antibody, Cy5 Conjugated (bs-7344R-Cy5) validated for, and at what dilution?
bs-7344R-Cy5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does OAS3 Polyclonal Antibody, Cy5 Conjugated (bs-7344R-Cy5) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat.
How should bs-7344R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise OAS3 Polyclonal Antibody, Cy5 Conjugated (bs-7344R-Cy5)?
KLH conjugated synthetic peptide derived from human OAS3
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-7344R-Cy5)?
Cy5 on bs-7344R-Cy5: excitation / emission 625,650nm/670nm.