Allergin-1 Polyclonal Antibody, APC-Cy7 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-7348R-APC-Cy7-100uL |
| Product Name | Allergin-1 Polyclonal Antibody, APC-Cy7 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human |
| Specifications | |
| Conjugation | APC-Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Allergin-1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Cell membrane |
| Synonyms | C17orf60;Chromosome 17 open reading frame 60. |
| Background | As a major event in type allergic reaction,degranulation of mast cells is triggered by the interaction between specific IgEs and high affinity IgE receptors (FcRIs) on mast cell membrane,and is followed by the release of a battery of inflammatory mediators,such as histamine,leukotrienes and arachidonic acid,and these inflammatory mediators in turn induce a series of allergic symptoms.Recently,Allergin-1 has been found to be an immunoglobulin-like receptor preferentially expressed on mast cells.The crosslinking of Allergin-1 to FcRIs on mast cells may inhibit the signal transduction from FcRIs to intracellular immunoreceptor tyrosine-based inhibitory motif (ITIM),generate ITIM inhibitory signals,which then participate in the regulation of mast cell degranulation,and finally block the initiation of allergic inflammation. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Allergin-1 Polyclonal Antibody, APC-Cy7 Conjugated (bs-7348R-APC-Cy7) validated for, and at what dilution?
bs-7348R-APC-Cy7 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-7348R-APC-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Allergin-1 Polyclonal Antibody, APC-Cy7 Conjugated (bs-7348R-APC-Cy7)?
KLH conjugated synthetic peptide derived from human Allergin-1
What are the excitation and emission wavelengths of the APC-Cy7 conjugate (bs-7348R-APC-Cy7)?
APC-Cy7 on bs-7348R-APC-Cy7: excitation / emission 650nm/780nm.