PGA3 Polyclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bs-7369R |
| Product Name | PGA3 Polyclonal Antibody |
| Applications |
WB
ELISA
IHC-F
IF
IHC-P
ICC/IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Pepsinogen I |
| Immunogen Range | 101-200/388 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C for 12 months. |
| Target | |
| Gene ID | 643834 |
| Swiss Prot | P0DJD8 |
| Synonyms | PEPA3_HUMAN; PGI; Pepsin A-3; Pepsinogen 3 group I; PEPSINOGEN I--SECOND LOCUS; Pepsinogen-3; PGA3. |
| Background | Pepsin is one of the main proteolytic enzymes secreted by the gastric mucosa. Pepsin consists of a single polypeptide chain and arises from its precursor, pepsinogen, by removal of a 41 amino acid segment from the N-terminus. Pepsinogen is synthesized in the stomach lining, and hydrochloric acid, also produced by the gastric mucosa, is necessary to convert the inactive enzyme and to maintain the optimum acidity (pH 1-3) for pepsin function. Pepsin is particularly effective in cleaving peptide bonds involving aromatic amino acids. Pepsin shows extremely broad specificity, and although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent. The amino acid composition of Pepsin C differs from those of pepsinogen and pepsin especially in the content of basic amino acids, glutamic acid, aspartic acid, leucine and isoleucine. |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
| IHC-F | 1:100-500 |
| IF | 1:50-200 |
| IHC-P | 1:200-400 |
| ICC/IF | 1:50-100 |
Frequently asked questions
Which applications is PGA3 Polyclonal Antibody (bs-7369R) validated for, and at what dilution?
bs-7369R is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-F (1:100-500); IF (1:50-200); IHC-P (1:200-400); ICC/IF (1:50-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PGA3 Polyclonal Antibody (bs-7369R) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-7369R be stored?
Store at -20°C for 12 months. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PGA3 Polyclonal Antibody (bs-7369R)?
KLH conjugated synthetic peptide derived from human Pepsinogen I Immunogen range: 101-200/388.